This study examined the assurance of quality assessment in the teaching of Business Education in Nigerian Colleges of Education. The study was limited to five Colleges of Education in the south-west zone of Nigeria using predetermined mechanism such as human resources e.g. lecturers and support staff, material resources, funding, facilities, promotions and staff development and use of computers in information storage, retrieval and teaching (electronic interactive board). Teaching methods such as team teaching, evaluation techniques involving: question pool, conference marking and external moderation were examined. One hundred (100) respondents were randomly selected for the study. These comprised of both male and female academic staff and students in the department of Business Education of the colleges. Three hypotheses were tested using the Chi-square analysis. Data for the study were collected through a modified self-developed questionnaire with the responses range of Agreed (A), strongly Agreed (SA), Disagreed (D) and strongly Disagreed (SD). The findings showed that significant quality or assurance mechanisms were assured in human resources and evaluations in the teaching of Business Education in the colleges of education at the south-west zone of Nigeria. On the basis of the findings, it was recommended that instructional materials enable learners to comprehend the interrelatedness of knowledge in different subject areas that have link with business education. Instructional materials enhance teacher-learner interaction in the classroom setting. Instructional materials concretize conceptual abstraction to solve problem in real life situation.